Workflow from Scientific Research

A cartoon illustrating the full methodological pipeline from antibody testing (first by immunofluorescence microscopy [IF] and then using IMC), staining and ablation of full sample cohort, automated image analysis using SIMPLI (see Methods) to final data analysis in R. Graphics were created in BioRender (Caramello, A. (2025) https://BioRender.com/z22okvn ) 103 .

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