SDS–PAGE and native PAGE immunoblotting of soluble and insoluble cell lysate fractions from an equivalent cell mass of CHO cells expressing indicated A1AT variants. Native PAGE immunoblotting was performed using antibodies recognizing total (a0409) and polymerized (mAB 2C1 ) A1AT. SDS–PAGE immunoblotting was performed using an N-terminal directed antibody against A1AT present in all variants (MA5-15521).
