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The details of the conversion strategies for sequencing of the native Illumina libraries on G4 and AVITI. For G4: the library is amplified in the presence of complementary primers containing G4 indexes and S1 and S2 adaptors which replace the flanking sequences from the kit. For AVITI: the library is circularized with the use of a splint oligo which anneals to the library, followed by rolling amplification to produce the RNA colony forming the basis of avidity sequencing

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