Effects of variable LAC12aGE machineries on cell proliferation, including: Neg., negative control transfected with Cas12a plasmid in the absence of crRNAHGF; Pos., positive control transfected with Cas12a plasmid and wt-crRNAHGF; UV, cells transfected with Cas12a plasmid/OFF-crRNAHGFin the absence of UV light; +UV, cells transfected with Cas12a plasmid/OFF-crRNAHGFin the presence of UV light. Panel I: Confocal microscopy images of HepG2 cells stained with Alexa Fluor 555 (Edu assay) and Hoechst (nucleus) dyes. Scale bar, 50 m. Panel II: Proliferation ratios of HepG2 cells corresponding to the experiments shown in panel I. The cell proliferation ratio (%) is defined as m/n 100%, where m corresponds to the number of Alexa Fluor 555 red fluorescent cells, and n is the total number of Hoechst blue fluorescent cells.
