Effects of variable LAC12aGE machineries on migration, including: Neg., negative control transfected with Cas12a plasmid in the absence of crRNAHGF; Pos., positive control transfected with Cas12a plasmid and wt-crRNAHGF; UV, cells transfected with Cas12a plasmid/OFF-crRNAHGFin the absence of UV light; +UV, cells transfected with Cas12a plasmid/OFF-crRNAHGFin the presence of UV light. Panel I: Wound healing assay corresponding to cell migration capacities of HepG2 cells. Scale bar, 100 m. Panel II: Changes in vacant area separating cells treated with the variable LAC12aGE configurations displayed in panel I.
