Effects of variable LAC12aGE machineries on apoptosis, including: Neg., negative control transfected with Cas12a plasmid in the absence of crRNAHGF; Pos., positive control transfected with Cas12a plasmid and wt-crRNAHGF; UV, cells transfected with Cas12a plasmid/OFF-crRNAHGFin the absence of UV light; +UV, cells transfected with Cas12a plasmid/OFF-crRNAHGFin the presence of UV light. Panel I: Confocal microscopy images of HepG2 cells stained with Annexin V-FITC (apoptosis evaluation) and Hoechst (nucleus) dyes. Scale bar, 20 m. Panel II: Cell apoptosis ratios of HepG2 cells corresponding to the experiments shown in panel I. The cell apoptosis ratio (%) is defined as x/y 100%, where x is the number of Annexin V-positive apoptotic cells, and y is the total number of Hoechst blue fluorescent cells. ***,P< 0.001. Data are presented as mean SD (n= 3).
