Bar Plot from Scientific Research

Panel I: Spatial control of LAC12aGE machinery using a photomasked pattern. Panel II: Confocal microscopy images corresponding to fluorescence of sfGFP and mCherry in HEK293T cells transfected with Cas12a/OFF-crRNAsfGFP. Regions i and ii, Cells in the absence of a photomasked pattern and without UV light activation. Region iii, Cells in the absence of a photomasked pattern exposed to UV light illumination. Region iv, Cells in the presence of a photomasked pattern exposed to UV light illumination. Scale bars, 100 m. Panel III: The relative ratios of sfGFP to mCherry derived from the integrated fluorescence intensities of sfGFP and mCherry displayed in panel II. ns, no significant difference; ***,P< 0.001. Data are presented as mean SD (n= 3).

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