Microscopy from Scientific Research

Sum intensity projections of ApoTome immunofluorescence images of NIH3T3 cells showing the localizations of endogenous rhotekin2 (RTKN2; red in merge) and cytochrome c (Cytc; mitochondrial marker; green in merge). The boxed area is shown at higher magnification as an inset in the respective image. The yellow line in the merge inset represents the line for showing an intensity profile histogram of fluorescence intensities of both channels, clearly demonstrating rhotekin2’s spatial overlap with cytochrome c (yellow in merges). Insets, enlargement of boxed area. Bar, 20 m.

DOI