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Immunoblotting analyses of coimmunoprecipitation experiments from lysates of HEK293 cells coexpressing Flag-RTKN2’ together with GFP-syndapin I (GFP-SdpI) and a short and long isoform of syndapin II (GFP-SdpII-s and GFP-SdpII-l), respectively. Specific coimmunoprecipitation of RTKN2’/syndapin complexes (green arrowheads) were obtained by anti-Flag antibodies but not by control IgGs. White lines/lanes indicate lanes omitted (h, i) and therefore are not accompanied with additional standards. All experiments shown as representative images (h, i, k, l) have been done at least twice with similar results.

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