Gel from Scientific Research

Open access visualization of Gel, Photo, Syndapin I Interaction, Y2H Screening, Reporter Gene Activity
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Verification of the syndapin I interaction of two prey clones (clone#336 and full-length clone#541) isolated by Y2H screening with full-length syndapin I as a bait via retransformation of isolated plasmids by reporter gene activity assessment (growth on quadruple drop-out plates and -Gal activity, respectively) demonstrating a SH3 domain-dependent syndapin I interaction. pGBTK7 served as negative control for the bait vectors.

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