Workflow from Scientific Research

Workflow demonstrating whole-proteome microarray screening and downstream selection of immunoreactive antigens. 1,296 B. afzelii proteins were printed on a microarray chip and probed with human sera from the discovery cohort (HC, EM, DIS, and PT sera) and murine cohort (PI, EI, LI, and AT sera) to identify immunoreactive antigens. Selection of antigens was based on pre-defined filtering criteria representing p-value, area under the curve, and positive mean increase in normalized signal intensities. Forty-eight common antigens and a total of 200 antigens with significant IgM and IgG responses were identified from both datasets.

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