(D) Percentages for each population among all live lin-cells. (A-D) Each data point represents the cells harvested from 1 well of 96. Graphs and table show mean +/- SEM; statistical centers are indicated by the SEM. n= 4 for each experiment, where n is an independent biological replicate performed on a separate day, with a total of nine technical replicates across n= 4. Statistical analysis was by unpaired t test or Mann-Whitney rank test based on the Shapiro-Wilk normality test. A linear regression model was used to plot thymocyte numbers over time (C). No p value indicates not significant (p> 0.05); *p< 0.05 and **p< 0.01. (A-D) Data shown are for live CD45+lineage-negative cells (lin = CD11b, CD11c, Gr-1, Nk1.1, B220, EpCAM, and Ter119); CD69+MHCI+ and CD69-MHCI+ data shown are after gating on TCR+cells. See also Figure S5.
