mTOs were generated with a constant number of TECs (1 x 105) and FTMs (4 x 104) and varying numbers of DN cells (D) or with and without thymic ECs (E). No EC control (-), 0 ECs, and ECs (+), 1-2 x 104 ECs. Plots show the absolute numbers of recovered thymocytes for each sub-set with the statistical center indicated by the standard error of the mean (SEM). (E) n= 8, where n is an independent biological replicate performed on a separate day. Cell numbers shown are per single well of 96 wells. Statistical analysis was by ordinary one-way ANOVA or Kruskal-Wallis rank test (C and D) or unpaired t test or Mann-Whitney rank test (E) based on the Shapiro-Wilk normality test.
