mTOs were generated with a constant number of TECs (1 x 105) and FTMs (4 x 104) and varying numbers of DN cells Control, 5 x 104 DNs; Hi DN, 2 x 105 DNs; and Lo DN, 1 x 104 DNs. Plots show the absolute numbers of recovered thymocytes for each sub-set with the statistical center indicated by the standard error of the mean (SEM). (D) n= 8, where n is an independent biological replicate performed on a separate day. Cell numbers shown are per single well of 96 wells. Statistical analysis was by ordinary one-way ANOVA or Kruskal-Wallis rank test (C and D) or unpaired t test or Mann-Whitney rank test (E) based on the Shapiro-Wilk normality test.
