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mTOs were generated with a constant number of TECs (1 x 105) and DNs (5 x 104) and varying numbers of FTM cells: Hi FTM, 4 x 104 FTMs per well (physiological FTM:TEC ratio); Lo FTM, 1.5 x 104 FTMs; and no-FTM mTO, 0 FTMs. ECs were not included. Representative plots showing thymocyte sub-set distribution after 14 days culture, for the markers and conditions shown. Data shown are for live CD45+lineage-negative cells (lin = CD11b, CD11c, Gr-1, Nk1.1, B220, EpCAM, and Ter119); CD69+MHCI+and CD69-MHCI+data shown are after gating on TCRβ+cells.
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