Quantification of fraction of larvae with regressed tails that have been treated with either DMSO (control), anoctamin inhibitor, and/or SERCA inhibitors. For (I)–(K), data are represented as boxplots, with the median and min/max values (whiskers) marked. For statistical analysis of data shown in these images, we performed Kruskal-Wallis tests (for all images,p< 0.0001), followed by Dunn’s multiple comparisons tests.