Immunostaining of PI(4)P and Syp in hippocampal neurons treated with or without TeNT under resting conditions and following chemical stimulation. Left: representative images of hippocampal neurons either non-stimulated () or chemically stimulated (+), after pretreatment with (TeNT) or without (CTRL) TeNT, and labeled with PI(4)P (red) and Syp (green) antibodies. Right: quantification of PI(4)P/Syp ratio with or without chemical stimulation and with or without TeNT pretreatment. Values were normalized to control neurons (n= 20/2).p< 0.0001 and n.s., not significant. One-way ANOVA with Tukey-Kramer post hoc test. Scale bar: 2 m. See alsoFigure S5.
