The indicated ER shapers (reticulon-4A is the only isoform of RTN4 shown for simplicity) were targeted by siRNA either alone or in combination with an additional siRNA duplex targeting PERK. 48 h later, cell subsets were mock treated with vehicle (DMSO) or exposed for 6 h to tunicamycin. An increase in the ER expansion ratio in tunicamycin-treated subsets, as compared to their DMSO-treated counterparts, is shown for each siRNA group. Red lines indicate the threshold of significance (p< 0.05) for the difference between single siRNA treatment and the PERK-ER shaper double siRNA combination. Highlighted targets (yellow frames) exhibit a response comparable to mock-silenced cells and rescue of the phenotype associated with PERK depletion. Data are derived from four biological replicates (~2,000 cells per well).
