MCF10A cells were exposed to tunicamycin for 4 h and then fixed and allowed to recover for indicated times in the presence or absence of guanabenz. Whole-cell extracts were subjected to western blot analysis for indicated markers (G); fixed samples were processed for immunostaining for calreticulin and analyzed for relative peripheral ER content. Data are derived from four biological replicates (~2,000 cells per well). All scale bars across micrographs represent 15 m. Statistical significance values from Student’s t tests are indicated as*p< 0.05,**p< 0.01, and***p< 0.005. n.s.p> 0.05. Bar graph items show mean values (bar graphs) and standard deviation (error bars); dot plots represent individual replica values, with their average indicated with an horizontal bar.
