CD4 + T cells were edited with 1G4-LY and CD8 SEEDs (+M3814) and immunomagnetically purified with BW242 and anti-CD4 ( n = 3 donors). Assessment of cytotoxic activity of CD4 + T cells against NY-ESO-1 + A375 target cells (technical triplicate; data shown from one of four donors). Nonedited T cells, dark gray; 1G4-LY SEED-edited, red; 1G4-LY SEED-edited; CD8 SEED-edited, blue. Data are displayed as the mean (bold line) ± s.e.m. (shaded area). Significance was assessed at 72 h. The significance in g , h was assessed using a repeated-measures one-way ANOVA and Turkey’s multiple-comparisons test.
