STED microscopy (calreticulin immunostaining) (E) and electron microscopy (F) of MCF10A cells across indicated conditions. Magnified cropped images show details of the indicated rectangular example regions of interest (ROIs) at perinuclear or peripheral cell regions. Pseudocoloring in (F): cyan, nuclei; red, mitochondria; yellow, ER.pvalues are indicated across experiments. Scale bars represent 10 μm in (E) and 5 μm in (F). Statistical significance values from Students t tests are indicated as∗p< 0.05,∗∗p< 0.01, and∗∗∗p< 0.005. n.s.p> 0.05. Bar graph items show mean values (bar graphs) and standard deviation (error bars); dot plots represent individual replica values, with their average indicated with a horizontal bar. Heatmap representsZscores as related to control average and standard deviation for each feature.
