RenillaNanoLuc PCA in HEK293T cells transfected with PKA subunits (positive control) or N-TAL1 and N-MYB, co-transfected with lenti-FLAG, lenti GATA3 WT or lenti GATA3 W329A. The mean of four independent biological replicates measured in triplicate is shown as SD,*p< 0.0001, unpaired t test. Representative western blot of MYB, TAL1, GATA3, and GAPDH in whole-cell extracts from HEK293T cells transfected with Small-Luc TAL1 and Large-Luc MYB, alongside FLAG-GATA3 WT or FLAG-GATA3 W329A;N= 2.
