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Schematic representation of the genome-wide CRISPR-Cas9 knockout screen. Jurkat GFP-TAL1 cells were transduced with the Brunello sgRNA library at low multiplicity of infection (MOI) and selected with puromycin for a 5 day period. Cells were sorted according to the GFP intensity signal as GFP-high, -middle, -low, and -negative populations. sgRNAs from GFP-low and GFP-middle cell populations were PCR amplified and analyzed by next-generation sequencing on an Illumina platform.

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