Gel from Scientific Research

SDS-PAGE gel image of secreted protein fractions from P. aeruginosa PAO1 and its mutant derivative xcpA (T2SS defective), both carrying a miniTn7 insertion to induce the expression of pap1 with the lacIq-Ptac system. The secreted protein fractions were obtained from cell-free culture supernatants of these strains after growing in the presence or absence of IPTG 1 mM for 18 h (as detailed in the STAR Methods). The red arrow indicates the band corresponding to Pap1. One representative image out of 3 biological replicates is shown.

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