Functional characterization ofGmGS11A,GmGS11B,andGmGS13A in planta. Photographs of soybean hairy roots under(a)white light and(b)UV light with a GFP filter indicating transgenic roots are shown.(c)GmGS11AandGmGS11Btranscript levels in silenced (Si) roots compared to control hairy roots analyzed using quantitative RT–PCR.(d)Glyceollin levels in GmGS11A-Si and GmGS11B-Si lines compared to control hairy roots.(e)GmGS13Atranscript levels in GmGS113A-Si roots compared to control hairy roots.(f)Glyceollin levels in GmGS13A-Si lines compared to control hairy roots.(g–i)Relative transcript abundance ofGmGS11A,GmGS11B, andGmGS13Ain OE transgenic lines.(j–l)Glyceollin levels in OE lines compared to control hairy roots. Relative transcript abundance and glyceollin concentration indicate the mean values of four biological replicates SEM. Asterisks indicate the statistical significance of thet-test. P< 0.05, P< 0.01, and ns, not significant (P> 0.05).
