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In vitroenzyme assays for glyceollin synthase activity. NE, no enzyme control; EVC, empty vector control. The enzyme/substrate combinations used in each reaction are indicated with the base peak atm/z321.112 observed in positive ion mode. WGE-treated roots show three glyceollins (m/z321.112) at their respective retention times in the order of elution: glyceollin III, II, and I.

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