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Schematic illustrating the experimental design: three sgRNAs targeting trmt1 exons were injected into one-cell-stage embryos to generate F0 knockouts. Sexually mature F0 knockouts were bred with WT fish, and the resulting F1 progeny were genotyped to identify inheritable mutant allele carriers. Positive F0 founder carriers were inbred to obtaintrans-heterozygous (−/−) F1 knockout progeny.

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