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2D free energy profiles of the inward-outward transition coupled to the Na + translocation with AE1 reduced ( left ) or oxidised ( right ). RC 1 distance between TM4 of RnfA and RnfE; RC 2 distance of Na + and the centre of mass of RnfA/E, projected onto the Z-axis (see Supplementary Figs. 18 20 for details).
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