Rnf complementation strain rescues the growth of A. woodii on H 2 and CO 2 . The Rnf strains containing mutation of residues involved in Na + binding and translocation showed either reduced growth or no phenotype. Mutation of residues involved in Na + pumping or gating (R67, L103), showed drastically reduced Fd:NAD + oxidoreductase activity as compared to the wildtype protein. Activities are the mean s.e.m. of three independent biological replicates, measured in triplicates ( n = 3).
