Dual-luciferase assays in the N. benthemiana leaves. GFP and RGSV P2 protein were used as negative controls. The Luciferase/Renilla luciferase (LUC/REN) ratio was calculated as the final transcriptional activity. Means SD were shown (n= 6). Data were analyzed by one-way ANOVA with Tukey’s test. Different letters indicate statistical significance (p< 0.05).