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Rnf complementation strain rescues the growth of A. woodii on H 2 and CO 2 . The Rnf strains containing mutation of residues that stabilise the cofactors could not recover growth on H 2 and CO 2 or show any Fd:NAD + oxidoreductase activity. Activities are the mean ± s.e.m. of three independent biological replicates, measured in triplicates ( n = 3).

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