Calcium signals in the rIPN of Cont (blue;n= 508 cells from 4 slices), Cont with nAChR blocker cocktails (Cont + NB) (pale blue;n= 607 cells from 6 slices), and cCKD (red;n= 1,000 cells from 9 slices) mice during tetanic stimulation (left). The traces are averaged for each condition. Error bars, mean ± SEM. Right panel represents the peak signal intensity during stimulation. Each dot indicates the peakΔF/Fof each cell of Cont, Cont + NB, and cCKD slices (H(2)= 49.14,p< 0.0001, Kruskal-Wallis test with Dunns multiple comparison test). Bars in (C), mean. Statistical significance was defined as∗p< 0.05 and∗∗∗p< 0.001.
