Scatter Plot from Scientific Research

Cholinergic dmsr-1 is required for sleep downstream of RIS. unc-17p::FLP:n= 25, dmsr-1[FRT exons 2-3 FRT]:n= 16, unc-17p::FLP; dmsr-1[FRT exons 2-3 FRT]:n= 23, with 3 biological replicates. The corresponding bout alignment can be found in Figure S3I. Alignment of RIS calcium activity peaks shows no significant changes in RIS transients in the conditional knockdown of dmsr-1 in cholinergic neurons compared with its controls. However, sleep was reduced by approximately 40% at the RIS transient peak in the knockdown.*p 0.05,***p 0.001. Wilcoxon rank-sum test was performed for the average GCaMP intensity and sleep fraction from 1 to 1 min and from 5 to 10 min.

DOI