dmsr-1(qn45) does not affect the frequency of RIS activation. We extracted all RIS activation transients during the 5-h recording, regardless of whether they were associated with sleep bouts. n.s. = not significant (p> 0.05), Wilcoxon rank-sum test. N2:n= 77,dmsr-1(qn45):n= 74 animals, with 3 biological replicates, animals were imaged for 5 h.