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Representative culture plates of crossings between theMAT1-1isolate D141 with AF293 [MAT1-2] (left) or AfS269 [MAT1-2;ppgB] (right) point inoculated on oatmeal agar after 6 months of incubation. Impaired cleistothecia formation for the D141 AF269 combination illustrates a decreased sexual capacity of theppgBdeletion mutant. White arrows indicate mature fruiting bodies formed in the D141 AF293 cross. Scale bars, 1 cm. See alsoFigure S4.

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