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Halo assay with the pheromone-sensitive yeast strain DBY7442 [MAT;sst2] expressing the presumed PreA pheromone receptor upon confrontation with supernatants (sn) from glucose- or xylose-supplemented cultures (GMM and XMM, respectively) of AfS147 [pxylP::MAT1-2-1] and AfS270 [pxylP::MAT1-2-1;ppgB]. The absence of a growth arrest halo for the latter grown in XMM indicates thatppgBencodes ana-factor-like pheromone that is sensed by the PreA pheromone receptor.
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