Microscopy from Scientific Research

AC-deficient TIR1 fails to mediate auxin-induced activation of DR5::Luc reporter. F1 seedlings of DR5::Luc cross with pTIR1::TIR1 , pTIR1::ccvTIR1 , pTIR1::ccvTIR1 ACm1 and pTIR1::ccvTIR1 ACm3 were treated with 200 nM cvxIAA. Images showed representative examples of DR5::Luc intensity after 4 h. The colour mode of Fire LUT was applied to the images. Quantification of the DR5::Luc intensity is showed in Extended Data Fig. 2a .

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