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Nondegradable Aux/IAA proteins (axr2 and axr3) fail to enhance auxin-induced AFB5 AC activity in vitro. cAMP quantification by liquid chromatography with tandem mass spectrometry were conducted in the reaction presence of 10 M IAA, AXR2/IAA7, AXR3/IAA17, axr2 and axr3 in the indicated combinations. Mean + s.d. from three biological replicates, t -test, one-sided.
#Bar Plot#Error Bars#Clustered#Scatter Plot#Aux/IAA Proteins#AFB5 AC Activity#cAMP Quantification#Liquid Chromatography#Tandem Mass Spectrometry#Biological Replicates
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