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Expression of stabilized axr3 prevents auxin-induced cAMP production in vivo. cAMP quantification in the XVE > >axr3-mCherry line. Seedlings were pre-treated with Mock or β-oestradiol for 4 h, followed by treatment with same medium containing 100 nM IAA for different time periods. Mean + s.d. from six biological replicates, t -test, one-sided.

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