SBS burden across HSCs (data as in c ), whole blood and individual colonic crypts in the three aged mice. Bar height represents mean SBS burden, and error bars denote 95% CI. Peripheral blood and colonic crypt somatic mutation burdens were measured with nanorate sequencing and WGS, respectively. HSC colony counts per sample are described in b ; nanorate sequencing results are from a single replicate per animal, and n = 6, 5 and 5 colonic crypts for M7180, M7181 and M7182.
