Mean fluorescence lifetime (TauM at photon-weighted center of mass of the corresponding phasor plot) of TDP-43mNLS-Clover in either (1) diffuse state (no seeds), (2) condensates (no seeds), or (3) aggregates (+ seeds), at 1 or 8 days post-seed exposure. Data points are individual cells from 3 experiments (different shapes represent separate experiments). Mean ± SD. Dunnett’s one-way ANOVA (∗∗p< 0.01,∗∗∗p< 0.001,∗∗∗∗p< 0.0001). See alsoFigure S4.
