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Diagram of the selection process of cgd6_40. Criteria included: (1) a predicted signal peptide, (2) a high non-synonymous/synonymous ratio (2 > dN/dS), and (3) highly expressed at sporozoite and asexual life cycle stages within publicly available transcriptome datasets (top 1% of RNA-seq by tpm). Schematic for epitope-tagging cgd6_40 at the genetic locus along with insertion of constitutively expressed NanoLuciferase reporter and neomycin resistance marker. Graph showing the relative luminescence reading from collected fecal samples during the initial transfection and selection of cgd6_40-HA transgenic parasites withinIfn-mice. Mean and SD for three technical repeats.
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