Gel from Scientific Research

Schematic overview of the permanganate DNA footprinting assay. The UREA-PAGE gel image used to generate the graph in (I) highlights KMnO4-dependent enriched bands for WTSpyCas9 (gray), SpG (pink), and SpRY (purple) in colored dashed boxes. The gel image was rendered in ImageLab 6.1 (BioRad) and cropped to exclude irrelevant neighboring lanes.

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