Illustration from Scientific Research

Schematic overview of the AuRBT experimental setup. dCas9 and dSpRY, both with similar active protein fractions (Figure S1H), were used at concentrations of 0.8 and 4 nM, respectively. sgRNA3 contains a 20-bp match to the Target1 sequence flanking an NGG site. is the measured rotor bead angle. All AuRBT experiments were performed under F = 7 pN of tension.

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