Distribution of gene-specific codon protection index for cells in YPD (median 0.26, red), in CSM (median -0.18, green), and simulated 100% out-of-frame decay (median -0.58, purple). Codon protection index was calculated as the ratio of the reads corresponding to the protected frame (F1) with respect to the average number of reads of the non-protected frames: log2(F1/((F2 + F0)/2)).
