Gel from Scientific Research

Illustration of the phospho-mimicking Stm1S229D, T248, S267D, S271D(Stm1S-T/D) and non-phosphorylatable Stm1S229A, T248A, S267A, S271A(Stm1S-T/A) (top) mutants. Serial dilution spottings (bottom) of stm1Δ cells with empty control plasmid or plasmids overexpressing Stm1 (WT), Stm1S-T/D or Stm1S-T/A on YPD media. Cells were treated or not (UT) with 2.0 or 2.5 nM rapamycin (Rap) and incubated (3 days, 30°C). Representative image from n =3 independent experiments.

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