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Naive wild-type and Arhgef1 -deficient CD8 + T cells were electroporated with nucleoprotein complexes of Cas9 and single guide RNAs (sgRNAs) targeting Tbxa2r or scrambled sgRNA control (Ctrl) and stimulated with anti-CD3/28 antibodies and rhIL-2 in the presence or absence of 5 μM TXA 2 analogue U46619. n = 5 independent replicates per condition.
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