Frequency of cells expressing TNF, IL-2 and IFN, presented in terms of cytokine polyfunctionality, following intracellular cytokine staining of CD8 + T cells from lungs of Cd4 cre (cWT, n = 11) and Arhgef1 fl/fl Cd4 cre ( Arhgef1 cKO, n = 10) mice 17 days after intravenous injection of B78ChOva melanoma cells.