Flow cytometry analysis of BAMs from total brains (without the dura mater) from adult Il34+/+, Il34LacZ/+, Il34LacZ/LacZ mice. (Left) High-dimensional analysis and UMAP visualization demonstrate three different BAM subsets (of Gr1-Mertk+CD64+F4/80hi cells), defined as CD163+(CD206+), CD206+MHC class II+(MHCII) and MHC class II+. Data were transformed and percentile normalized. (Right) The heatmap shows the mean marker expression of markers by the respective BAM subsets. Data are normalized per column. Total counts for the different BAM subsets are derived from the UMAP. Number of cells (BAMs) per genotype used for the UMAP: Il34+/+, 9,116; Il34LacZ/+, 5,522; Il34LacZ/LacZ, 1,529. n= 5 per genotype from 1 experiment.
