Flow cytometry analysis of brain macrophages isolated from Il34LacZ/+andIl34LacZ/LacZmice at E18.5, P5, P7, P14, and adulthood. Representative FACS plots for microglia and BAMs are shown, and cell numbers are displayed as percentages normalized to Il34LacZ/+mice.n= 4-9 from 3-4 independent experiments. In all experiments (A-D), microglia and BAMs (pregated on CD45+Ly6C-Ly6G-CD64+MerTK+) were respectively gated on CX3CR1+CD206-and on CX3CR1+CD206+in mice ≤ P7 and on CD11bhiF4/80+and CD11b+F4/80hior CX3CR1+F4/80+and CX3CR1+F4/80hiin mice > P7. Statistical analysis was performed using Student’s t tests with Welch’s correction.*p< 0.05;**p< 0.01;***p< 0.001;****p< 0.0001; ns, not significant. Related toFigure S1.
